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Day 2, June 11（Tue.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 2P-36
Investigation and Structural Estimation of Xylose Containing Steviol Glycosides of Stevia (Stevia Rebaudiana) Leaves
(1Suntory Foundation for Life Sciences, 2Suntory Holdings, 3Suntory Beverage & Food, 4Suntory Global Innovation Center)
oTakehiro Watanabe1, Kohki Fujikawa1, Soichiro Urai2, Kazunari Iwaki3, Tadayoshi Hirai4, Katsuro Miyagawa4, Hiroshi Uratani1, Tohru Yamagaki1, Koji Nagao3, Yoshiaki Yokoo3, Keiko Shimamoto1
Some steviol glycosides obtained from Stevia rebaudiana leaves exhibit sweetness that is nearly 250-300 times higher than that of sucrose and they are therefore utilized as a low-calorie sweetener in the food industry. Among them, the sweetness of major glycosides composed of glucose residues (e.g., stevioside and rebaudioside A), in which all residues consist of glucose, has been widely studied. However, the properties of minor natural products containing rhamnose (Rha) or xylose (Xyl) residues are poorly investigated. In this study, we report investigation and structure estimation method to identify trace amounts of highly glycosylated steviol containing Rha or Xyl in stevia leaves and to verify what taste qualities they individually impart. First, elution trends of LC separations of available authentic samples and MS/MS fragment pattern analysis are performed. Next, stevia leaf extract was analyzed by LC-MS/MS. As a result, 18 peaks corresponding to steviol glycosides were obtained, and the structures of five new steviol glycosides were estimated, naming those containing Rha the "dulcoside" series and those containing Xyl the "rebaudioside FX" series.