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Poster Presentations
Day 2, June 11（Tue.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 2P-32
4D-LC/MS: Automated System for Peptide Mapping of mAb Charge Variants with Online Enzymatic Digestion
(Agilent)
oAkio Hayashi, Atsuko Naito
Protein biopharmaceuticals have emerged as important therapeutics for the treatment of various diseases including cancer, cardiovascular diseases, infection, inflammatory, and autoimmune disorders. Together with a huge therapeutic potential, these molecules come with an enormous, analytically demanding structural complexity. A key technology to study cha0rge variants that might arise from PTMs such as asparagine deamidation, -C-term lysine truncation, N-term cyclization (pyro-Glu formation). CEX buffers are typically composed of nonvolatile constituents, making these methods incompatible with MS. We show that an automated online 4D-LC/MS integrating CEX (1D), peak collection, 2D desalting and reduction, 3D Lys-C digestion, and 4D C18 LC/MS-based peptide mapping can be executed in 120 min a fraction for the in-depth characterization of mAb charge variants.