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- Plenary Lectures
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- Award Lecture
- Symposium Sessions（Day1, Day2）
- Fundamental Sessions（Day1, Day3）
- Young Researchers' Sessions (Int'l)（Day1, Day3）
- Young Researchers' Sessions（Day1, Day3）
- Poster Presentations（Day1, Day2, Day3）
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Poster Presentations
Day 2, June 11（Tue.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 2P-31（3B-O1-0915）
Development of a Proteomics Separation Platform Using HILIC/MS/MS
(1Kyoto Univ., 2NIBIOHN)
oEisuke Kanao1,2, Koshin Akamatsu1, Jun Adachi1,2, Yasushi Ishihama1,2
Hydrophilic Interaction Chromatography (HILIC) coupled with liquid chromatography/tandem mass spectrometry (LC/MS/MS) is a promising technique for bottom-up proteomics. HILIC utilizes a high organic content solvent for the mobile phase, improving the MS sensitivity and throughput. Despite the potential, challenges in peptide separation robustness hinder practical application. To address this, a Two-step dissolution method was developed, optimizing peptide solubility with 25% ACN followed by adjusting to a final 95% ACN concentration. This approach significantly enhanced robustness, leading to the identification of over 1,200 proteins—20% more than conventional methods. The Two-step dissolution method demonstrated high reproducibility, suggesting its potential to improve the robustness of HILIC/MS/MS-based bottom-up proteomics.