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Poster Presentations
Day 2, June 11（Tue.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 2P-26
13C-metabolic Flux Analysis for Budding Yeast in Low Temperature Environment Using GC-MS
(Osaka Univ.)
oRyo Ishikawa, Souta Nakaya, Nobuyuki Okahashi, Fumio Matsuda
13C-metabolic flux analysis was performed using GC-MS to measure the metabolic state of budding yeast in a low-temperature environment. Yeast cells were cultured at 10, 13, 15, 30℃ to examine the metabolic changes with the temperature conditions. The specific growth rate, glucose uptake rate and ethanol secretion rate of yeast cultured at 10℃ were notably reduced compared to those at 30℃, 13C-metabolic flux analysis at 10℃ showed the rewiring of central carbon metabolism.