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Poster Presentations
Day 2, June 11（Tue.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 2P-17
A Novel Automated, Standardized, Kit-Based Sample Preparation Workflow for Bioanalytical Quantification of Therapeutic Oligonucleotides
(1Nihon Waters, 2Waters)
oMotoji Oshikata1, Mamiko Imaoku1, Nikunj Tanna2, Mary Trudeau2, Margot Lee2
Oligonucleotide Therapeutics (ONTs) are a key modality for drug development today given their powerful ability to address disease biology at the level of gene transcription and translation, and for their high target specificity and low toxicity. As the pipeline for ONTs continues to expand, so does the need for sensitive, accurate, and robust bioanalytical assays to support this drug development. LC-MS is a widely accepted technology for bioanalysis because of the performance of the technologies (i.e., sensitivity, selectivity, and linear dynamic range). However, the greatest source of variability for these assays arises from the sample preparation, and this is especially true for oligonucleotide. To this end, a simple, broadly applicable sample preparation workflow for ONTs that reduces the need for method development and brings greater consistency and reproducibility to LC-MS bioanalytical results is therefore highly desired. The OligoWorks™ SPE Microplate Kit (OligoWorks Kit) from Waters has been designed with this in mind. This work uses the OligoWorks Kit components and standard protocol automated on the Andrew+ Pipetting Robot to successfully extract a diverse range of ONTs from plasma and achieve accurate, robust, and reproducible bioanalytical performance, with little to no need for method development.