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Poster Presentations
Day 2, June 11（Tue.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 2P-08
Targeted DESI-MSI in MRM Mode Enables Higher Sensitivity and Specificity for Fast Quantitative-MSI of Chloroquine in Mouse Kidneys
(1HUSM, 2IMC, HUSM, 3SMA, 4Preppers Co. Ltd., 5NU, 6Waters)
oMd. Muedur Rahman1,4, Mstsayela Afroz1, Md. Al Mamun1,4, Takumi Sakamoto1,4, Ariful Islam4,5, Thanai Paxton6, Sato Tomohito1, Tomoaki Kahyo1,2, Yutaka Takahashi1,4, Mitsutoshi Setou1,2,3
Mass spectrometry imaging (MSI) is a powerful technique to visualize the spatial distribution of biomolecules and drugs. Compared to MALDI-MSI and SIMS, desorption electrospray ionization (DESI) has the advantage of being a soft and ambient ionization technique ideally suited to quantitative-MSI (Q-MSI). However, there are some challenging issues related to extraction efficiency, matrix effect, sensitivity, and specificity. In this study, targeted DESI-MSI using the multiple reaction monitoring (MRM) mode was applied to address the specificity and sensitivity issues. A tandem quadrupole mass spectrometer (Xevo TQ-XS, Waters, USA) coupled with a DESI source (DESITM XS, Waters, USA) was used to quantify the chloroquine (m/z 320.2→247.1) as a model drug in mouse kidney tissue. The calibration curve based on a mimetic tissue model was created to quantify the distribution of the drug. The detection of chloroquine using higher sensitivity, specificity, and scan rate is demonstrated in mouse kidneys after 2 hours of drug injection at a dose of 16.7 mg/kg body weight. This study provides some valuable insights into using DESI-MSI in MRM mode for the Q-MSI study of drugs and metabolites.