- Timetable
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- Plenary Lectures
- MSSJ Special Program
- Award Lecture
- Symposium Sessions（Day1, Day2）
- Fundamental Sessions（Day1, Day3）
- Young Researchers' Sessions (Int'l)（Day1, Day3）
- Young Researchers' Sessions（Day1, Day3）
- Poster Presentations（Day1, Day2, Day3）
- Evening Workshop
- Corporate Program
Poster Presentations
Day 1, June 10（Mon.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 1P-33（1C-O1-1451）
Investigation of Performance of Machine-Gun Proteomics on Consecutive 5,000 Analysis
(1Kyoto Univ., 2NIBIOHN, 3RIKEN IMS)
oAyana Tomioka1, Ryota Tomioka1, Eisuke Kanao1,2, Kosuke Ogata1, Koshi Imami3, Naoyuki Sugiyama1, Yasushi Ishihama1,2
Bottom-up proteomics using nanoLC/MS/MS has become widely used in clinical applications and in the elucidation of biological function, but balancing its throughput with robustness, sensitivity, and analytical depth remains a challenge. Increasing flow rate and shortening gradient time have been examined to improve throughput, but often result in lower sensitivity and lower depth of analysis than standard nanoLC/MS/MS. Here, we focused on capillary-flow LC to maintain sensitivity and developed an ultrahigh-speed LC/MS/MS that achieves 1.4 minutes per run, equivalent to 1000 samples per day. The method was shown to be remarkably robust, capable of more than 5,000 continuous measurements per week, including maintenance time.