- Timetable
- Download all abstracts
- Plenary Lectures
- MSSJ Special Program
- Award Lecture
- Symposium Sessions（Day1, Day2）
- Fundamental Sessions（Day1, Day3）
- Young Researchers' Sessions (Int'l)（Day1, Day3）
- Young Researchers' Sessions（Day1, Day3）
- Poster Presentations（Day1, Day2, Day3）
- Evening Workshop
- Corporate Program
Poster Presentations
Day 1, June 10（Mon.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 1P-28
Analysis of Extracellular Secretory Proteins in Neural Stem Cells Derived from Human Induced Pluripotent Stem Cells by Data-Independent Acquisition LC/MS/MS
(Yokohama City Univ.)
oMei Mikami, Yukako Kawachi, Daisuke Takakura, Nana Kawasaki
Induced pluripotent stem cell (iPSC) derived products have potential applications in regenerative medicine. For their clinical application, appropriate and efficient quality control during the manufacturing process is important. Currently, cell quality control is mainly based on the expression of intracellular molecular markers, which requires consumption of cell samples and complex manipulations. For efficient quality control, extracellular secretory proteins are useful. However, analysis of extracellular secretory proteins in culture supernatants is difficult. This is because extracellular secretory proteins are small amounts and are difficult to distinguish from proteins derived from medium. Recently, extracellular vesicles (EVs) have attracted attention as a non-invasive biomarker. EV enrichment enables distinguish from proteins derived from medium. Data independent acquisition mass spectrometry (DIA-MS) is suitable for the analysis of small amounts of proteins. Therefore, we developed a method to analyze extracellular secretory proteins focusing on extracellular vesicles by DIA-MS. Using this method, we identified extracellular secretory proteins specific to neural stem cells (NSCs) derived from human iPSCs. Many of them are involved in axon guidance and Wnt signaling pathway, reflecting the cellular state of NSCs.