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- 1P-04
Evaluation of Ions Produced by NanoESI in Native Mass Spectrometry Using Thermometer Ions
(1Yokohama City Univ., 2AIST)
oMichiko Tajiri1, Kazumi Saikusa2, Takahisa Ikegami1, Daiki Asakawa2, Satoko Akashi1
Native mass spectrometry (native MS) is a method that uses neutral volatile solvents to ionize protein complexes formed by weak non-covalent interactions, which can then be analyzed by mass spectrometry. Smaller sprayed droplet sizes are considered to result in softer ionization, and nanoelectrospray ionization (nanoESI) is used for native MS. In this study, nanoESI MS was performed under both denaturing and native conditions utilizing benzylammoniums, recently identified as suitable thermometer ions for estimating the internal energy distribution of ions produced by ESI. By comparing the internal energy distributions observed under these two conditions, ions produced by native MS were evaluated and discussed. Furthermore, the internal energy distribution of the ions, which varies with droplet size under native conditions, was quantitatively investigated.