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Poster Presentations
Day 1, June 10（Mon.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 1P-03
Rapid Determination of Free Fatty Acids in a Microtissue Sample Using Supercritical Fluid Extraction/Supercritical Fluid Chromatography-Medium Vacuum Chemical Ionization Mass Spectrometry.
(1Osaka Univ., 2MS-Cheminformatics)
oToshinobu Hondo1,2, Yumi Miyake1, Michisato Toyoda1
Medium vacuum chemical ionization (MVCI) mass spectrometry (MS), a novel technique developed in our laboratory since 2021, utilizes oxonium and hydroxide ions generated from the water via corona discharge for chemical ionization. A great advantage of MVCI is orders of magnitude higher detection sensitivity over electrospray ionization. MVCI reaction is carried out in approximately 100 Pa, readily connected with supercritical fluid chromatography (SFC). Fat-soluble molecules can be directly extracted by supercritical fluid extraction (SFE) from a small section of tissue or a cell placed on a frit, which can be introduced into SFC using hydraulics. The synergistic combination of high peak resolution in SFC and the elevated ionization efficiency of MVCI significantly enhances molecule identification performance.