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Day 1, June 10（Mon.） 14:51-15:09 Room D (Conference Room 202)
- 1D-O1-1451
Development of an Analytical System for Functional Metabolomics
(1Kyushu Univ., 2Osaka Univ.)
oYoshihiro Izumi1, Noriyuki Tomiyasu1, Masatomo Takahashi1, Taihei Torigoe1, Keisuke Nakata1, Kazuki Ikeda1, Kohta Nakatani1, Kosuke Hata1, Sho Yamasaki2, Takeshi Bamba1
Recent advances in liquid chromatography high-resolution tandem mass spectrometry (LC/HRMS/MS) have enabled the detection of thousands of molecular weight-related ion peaks. However, less than 20% of these peaks can be identified as metabolites. Therefore, the remaining 80% of unidentified ion peaks may contain functional metabolites that regulate human biological systems. The innate immune cells have immunoreceptors [e.g., Toll-like receptors, C-type lectin like receptors (CLRs)] that recognize a variety of exogenous and endogenous ligands, including carbohydrate, lipid, and protein components of pathogens and self. Although the identification of non-self or self ligands recognized by immune sensors is an important research topic, the identification of immune receptor ligands has had to rely on individual, one-time, and serendipitous discoveries due to technical limitations, such as analytical detection sensitivity, structure determination accuracy, and interaction detection sensitivity. In this study, we report the development of an efficient functional metabolite search method by integrating a metabolite preparative and analytical system based on LC/HRMS/MS with bioassays, using the search for novel lipid ligands of CLRs as an example.