- Timetable
- Download all abstracts
- Plenary Lectures
- MSSJ Special Program
- Award Lecture
- Symposium Sessions（Day1, Day2）
- Fundamental Sessions（Day1, Day3）
- Young Researchers' Sessions (Int'l)（Day1, Day3）
- Young Researchers' Sessions（Day1, Day3）
- Poster Presentations（Day1, Day2, Day3）
- Evening Workshop
- Corporate Program
Fundamental Sessions
Day 1, June 10（Mon.） 14:15-14:33 Room D (Conference Room 202)
- 1D-O1-1415（1P-24）
Development of a Targeted Lipidmics Method for EVs Using UHPLC-MS/MS
(1Teikyo Univ., 2Nippon Boehringer Ingelheim, 3Teikyo Univ. Hosp. Mizonokuchi, 4Teikyo Univ., 5Tohoku Univ., 6Tohoku Univ.)
oYuko Iwasaki1, Tomoko Fukuuchi1, Noriko Yamaoka1, Akira Togashi2, Noriyuki Matsutani3, Nobuhiko Seki4, Kuniyasu Niizuma5, Daisuke Saigusa1,6
It has been suggested that proteins, nucleic acids and lipid species contained in extracellular vesicles (EVs) differ depending on the onset and progression of disease. However, current lipidomics analysis is insufficiently precise, and the establishment of an accurate quantitative analysis method is required. We therefore developed an EVs targeted lipidomics method using UHPLC-MS/MS. Then, EVs targeted lipidmics analysis by UHPLC-MS/MS successfully detected lipid molecular species including ceramides and phospholipids, lysophospholipids, ceramides, hexosylceramides, cholesterylesthers, and triglyceride. Our targeted lipidmics may utilize to the biomarker discovery for plasma derived EVs and demonstrate EVs’ related disease mechanism.