Abstract

Lunchtime Seminars

Day 1, June 10（Mon.）　12:00-13:00　Room D (Conference Room 202)

1.Introduction to highly accurate microbial metabolomics using ZipChip CE-MS and its application
○Yuto Fukuyama, Shigeru Shimamura, Takuro Nunoura （JAMSTEC）

A ZipChip CE system is a microfluidic CE device providing quick separation and sensitive detection owing to nano-electrospray ionization from underivatized and tiny amounts of analytes (e. g. amino acids). Combining the ZipChip CE system and the Orbitrap Fusion Tribrid mass spectrometer enable us to improve sensitivity and accuracy of microbial metabolomics. In this seminar, we will introduce development of a novel 13C tracer-based metabolomics and its application on a hydrogenotrophic methanogen to identify metabolic pathway. The novel method requires only 1 μL of 100 nmol/L amino acids, which corresponds to those from approximately 1 × 104-105 prokaryotic cells. Total run time, including washing steps, is 15 minutes for each sample.

2. Attempt at “GPC-MALDI” using LC-CollectIR and high-resolution MALDI-TOFMS JMS-S3000 SpiralTOF™-plus 3.0
〇Takaya Sato (JEOL)

3. Introduction of LC/MS analysis under non-volatile buffer conditions using online desalting tubes and automatic online desalting tube switching device
〇Yutaka Takahashi (Preppers)

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