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Day 1, June 10（Mon.） 14:15-14:33 Room C (Conference Room 201)
- 1C-O1-1415（1P-32）
Protein N-terminomics by Tandem Mass Spectrometry with Electron Activated Dissociation
(1Kyoto Univ., 2AB SCIEX, 3NIBIOHN)
oKosuke Ogata1, Yuto Taniguchi1, Takeshi Shibata2, Yasushi Ishihama1,3
In this study, we evaluated peptide fragmentation using electron activated dissociation (EAD) to enhance the identification efficiency of peptides lacking basic functional groups. These peptides can be generated from protein N-terminus through LysargiNase digestion. Through the analysis, we identified 1124 N-terminal peptides using conventional collision induced dissociation (CID) and 1084 N-terminal peptides using EAD. There were 680 peptides that overlapped between the two datasets. By comparing the identification scores of 627 peptide ions with the same peptide sequence and precursor charge, we found that the average score was 23.6 for CID and 73.5 for EAD. Notably, for all peptides lacking basic functional groups, the scores obtained through EAD were higher than those obtained through CID, demonstrating the superiority of EAD.