The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Society Featured Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day3)

Workshops

Lunchtime Seminars (Day1, Day2, Day3)

Oral Sessions

Day 2, May 20（Thu.）　11:40-12:00　Room B (Zoom)

2B-O7-1140　　PDF

Analysis of the Changes in Glycoprotein Production and Their N-Glycosylation during Neural Differentiation of Human iPS Cells

(Yokohama City Univ.)
Kazumasa Kimura, Takaya Urasawa, Takumi Koizumi, Daisuke Takakura, Yuki Ohta, oNana Kawasaki

To understand the quality attribute of human-induced pluripotent stem cells-derived neuronal cells, the changes in glycoprotein production and their N-glycosylation during neuronal differentiation was analyzed by a sequential LC/MS/MS of cellular tryptic digest, enriched glycopeptides, and deglycosylated peptides. Our results and proposed method could provide valuable information for the development of processes and quality control strategies in the development of cell products.