The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Society Featured Lecture

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day3)

Workshops

Lunchtime Seminars (Day1, Day2, Day3)

Oral Sessions

Day 2, May 20（Thu.）　10:00-10:20　Room B (Zoom)

2B-O7-1000　　PDF

Glycoprotein Analyses by Mass Spectrometry

(1AIST, 2Hokkaido Univ.)
oHiroyuki Kaji1, Azusa Tomioka1, Takahiro Hiono1,2, Michihito Sasaki2, Yasuko Orba2, Hirofumi Sawa2, Atsushi Kuno1

Antigen determining blood types like ABO-type or Lewis-type is glycans attached on protein or lipid. Thus, in case of injectable medications such as vaccine, biopharmaceuticals, or viral vector, especially they were produced from non-human organisms or their cells, immunogenicity due to glycan is requested to test strictly. As an approach to analyze glycans on protein, we developed 2 methods employing mass spectrometry. One is a method for large-scale mapping of glycosylation sites, called IGOT, and another is to assign site-specific glycan compositions of a large number of proteins or in-depth for single protein, the method is named Glyco-RIDGE. In this talk, approach and applications of the methods will be presented.