Poster Presentations
- Day 3, May 13(Wed.) Poster (1008/09)
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3P-29 PDF
Application of cyclic ion mobility coupled to mass spectrometry for high peak capacity analysis of native and deuterated peptide mixtures
A major bottleneck for the analysis of peptides originating from protein digests is the requirement for long chromatographic separation times. The introduction of hybrid ion mobility-TOF mass spectrometers has proven advantageous in retaining peak capacity when chromatographic times are shortened, due to the extra dimension of separation afforded by the ion mobility (IM) device. Recent advances in travelling-wave IM instrumentation have led to the development of cyclic IM (cIM), a technology that provides variable IM separation by simply increasing the number of passes, or cycles, before MS detection. We investigated the utility of cIM for peptide separation under fast chromatographic conditions and by direct infusion. In addition, we monitored the retention of deuterium by exchanged peptides within the cIM device.