Abstract

Poster Presentations

Day 3, May 19(Fri.)  Room P (Multi-purpose Hall)

Development of a method for the analysis of free oligosaccharides in human plasma using MALDI-TOF MS and sialic acid linkage-specific alkylamidation

(1TMIG, 2Shimadzu, 3Keio Univ.)
oHiroki Tsumoto1, Takashi Nishikaze2, Yukiko Abe3, Yasumichi Arai3, Sadanori Sekiya2, Shinichi Iwamoto2, Koichi Tanaka2, Yuri Miura1, Tamao Endo1

We analyzed free oligosaccharides (fOSs) in human plasma by a combination of sialic acid linkage-specific alkylamidation (SALSA) and MALDI-TOF MS. Using this method, 78 peaks corresponding to fOSs containing high mannose- and complex-type fOSs were detected.