3P-28 PDF
Development of high sensitivity CE-MS metabolome analysis system using high resolusion mass spectrometer
Capillary electrophoresis-mass spectrometer (CE-MS) has been widely applied in metabolomics. High resolution mass spectrometer (HRMS) has not been yet up to fully fledged application for CE-MS in spite of the recent advance of HRMS. In this study, we developed a novel adapter for electrospray ionsource (ESI) that converted from high voltage applied nebulizer to grounded nebulizer. We performed metabolome analysis by using high-sensitive CE-HRMS system that combined CE with HRMS. We targeted primary metabolites (amino acids, peptides, nucleobases, organic acids, phosphate compounds and other ionic metabolites). Our developed system of CE-HRMS was able to improve 10-fold in detection sensitivity compared with CE-TOFMS system.