Abstract

Oral Sessions

Day 3, May 19（Fri.）　10:10-10:30　Room C (101)

3C-O1-1010　　PDF

The MS sensorgram of Protein-small molecules interaction using MIK-MS(Molecular Interaction & Kinetics Mass Spectrometry).

(¹Nissha Printing Co., Ltd., ²Biosys Tech., ³St. Marianna University School of Medicin, ⁴Silicon Kinetics Inc., ⁵School of Engineering, The Institute of Medical Science, and Drug Discovery Initiative, The University of Tokyo, ⁶Radioisotope Center, The University of Tokyo, Research Center for Advanced Science and Technology)
^oNaoko Obi¹, Tetsuya Fukuda², Noboru Nakayama², John Ervin⁴, Yasuhiko Bando², Toshihide Nishimura^2,3, Toshiaki Somehara¹, Satoru Nagatoishi⁵, Kohei Tsumoto⁵, Takeshi Kawamura⁶

Molecular Interaction & Kinetics Mass Spectrometry (MIK-MS) would be a powerful tool to screen drug candidates by integrating MS with nano Pore Optical Interferometry (nPOI). nPOI can measure the association and dissociation rates of biomolecules without labels, like Surface Plasmon Resonance. Since nPOI has a much larger ligand capture capacity, it can be used to directly detect analytes using a nano ESI LC-MS backend, thereby confirming the binding and the analyte. Here we will report that obtained MS sensorgram and calculated Koff ranking of the intermolecular interaction between Carbonic Anhydrase II and multiple small molecules using MIK-MS.