2P-28 PDF
MALDI-MS-based High-throughput Metabolite Profiling of Human Body Fluids
Generally, it is known that there is no perfect MALDI matrix for ionizing all molecules, and ionizable molecular coverage of each matrix is limited. To obtain favorable MALDI matrix for detecting low-molecular-weight compounds from biofluids, we performed the matrix screening using more than 50 synthesized matrix library and commercially available matrix. As a result, 1,5-diaminonaphthalene (1,5-DAN) and synthesized matrix No. 19 (quinolone derivative) were found as favorable matrices for detecting several biofluid metabolites. For high-throughput metabolite profiling, not only short analysis time but also high-throughput sample preparation method is important. We first investigated optimal sample preparation method (dilution rate of specimen, concentration of matrix, solvent of specimen and matrix). Then, we could find the best condition of sample preparation for high-throughput metabolite profiling of human biofluid by MALDI-MS. In this experiment, MALDI-MS analysis time is about 30 second and about 50 metabolite-derived peaks were obtained in a single run. The PCA score plots of obtained MS dataset from biofluids showed clear separation of clusters of healthy volunteers and diseases (breast and prostate cancer). Detailed results will be discussed in the session.