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Identification of actinomycetes by MALDI-TOF MS
Actinomycetes are important producers for biologically active secondary metabolites including antibiotics in the pharmaceutical industry. In recent years, MALDI-TOF MS has emerged as rapid and high throughput tool for the identification of microorganisms. It is very useful if the MALDI-TOF MS could be adapted to the screening of the actinomycetes. However, the standard MALDI-TOF MS typing method is unsuitable for actinomycetes due to the low extraction efficiency of the proteins and the low reproducibility of MS spectra derived from their life cycle complexity. We optimized extraction method and culture condition for them. We previously reported that the MALDI-TOF MS could be discriminate actinomycetes at the species level, focusing on Micromonospora species isolated from deep-sea sediments and soil samples. In this study, we report the capability of MALDI-TOF MS to discriminate novel species and the relationship among MS spectra ,16S rRNA gene, gyrB gene sequences and metabolic profiles.