1P-26 PDF
Development of an anionic metabolome analysis method using capillary ion chromatography/mass spectrometry
Recently, ion chromatography (IC) coupled with MS has been applied in metabolomics as it was found to be an excellent platform for separation of charged compounds. In this study, we have demonstrated the applicability of capillary IC/MS for anionic metabolome analysis. Under optimized condition, 49 anionic metabolites, including organic acids, sugar phosphates, nucleotides and coenzymes, were successfully separated and detected with mass spectrometer. Notably, the concentration detection limits of the tested compounds were between 1 and 500 nmol/l despite small volume injection (0.4 μl). The developed capillary IC/MS platform could be a powerful new tool for anionic metabolome analysis.