演題概要

ポスター発表

第３日　６月１９日（金）　　P会場

3P-39　　PDF

GC/MSによる定量メタボロミクスに向けたマトリックス効果の検討

(¹ジーエルサイエンス・ ²阪大院工・ ³理研・ ⁴九州大)
^o宮川浩美¹・ 湯面郁子²・ 鈴木智志¹・ 津川裕司^2,3・ 古野正浩¹・ 馬場健史^2,4・ 福崎英一郎²

There are some serious problems in quantitative analysis for metabolomics using gas chromatography / mass spectrometry (GC/MS), which are causing of the stability of sample and standard solutions including the responsive to trimethylsilyl (TMS) derivatization, and the matrix effects on MS ionization, and others. We measured the matrix influence on MS intensity during the comparison of standard solutions and extracts from yeast and serum. The effect of enhancement was observed on organic acids, sugars and nucleic acid bases, although the results of amino acids must be studied in detail.