演題概要

ポスター発表

第3日 6月19日(金)  P会場

ポリマー系HILICカラムを用いたリン酸基またはスルホ基を有する生体試料中低分子物質のLC/MS測定

(昭和電工)
o貴家潤治酒井聡子近藤英幸

In this study, a new HILIC column (HILIC or RP + anion exchange) Shodex® HILICpak® VT-50, packed with polyvinyl alcohol base material modified with quaternary ammonium functional groups, was used to develop high-sensitive LC/MS method for the quantification of various anionic low molecular substances. Phosphorylated saccharides such as Glucose-6-phosphate are important intermediates involving energy metabolism. In order to simplify conventional analytical method required derivatization or ion pair reagent, a new LC/MS method was studied. We found that VT-50, with aqueous solution including ammonium formate /acetonitrile as the eluent, was useful for this analysis. Peak shapes of phosphorylated saccharides were sharp with effective separation, and the calibration curves were linear. Both detection limits were 0.005 μM. Moreover, it was demonstrated that this method was applicable for various other anionic substances (e.g. cAMP, AMP, Taurine, Taurocholic acid). The new HILIC column was useful for the LC/MS analysis of various anionic substances using more simple conditions than previous methods.