演題概要

ポスター発表

第3日 6月19日(金)  P会場

ターゲットリン酸化プロテオミクスによる大規模キナーゼプロファイリング

(京大院薬)
o矢崎達也高橋知里若林真樹杉山直幸石濱泰

Phosphorylation is an essential regulatory mechanism to control various cellular functions. Quantitative phosphoproteomics is a powerful tool to monitor the entire map of cellular phosphorylation of proteins, in order to understand the comprehensive biological functions. In this study, we targeted kinase-derived phosphopeptides including phosphosites which are known to regulate the kinase activity. By quantifying these phosphopeptides using parallel reaction monitoring LC-MSMS, we successfully profiled 50 kinases in human cancer cells treated with various molecular-targeting drugs.