3P-24(3C-O1-0950) PDF
経皮投与型薬剤の動態分析に向けたレーザーイオン化イメージング質量分析手法の開発
Transcutaneous vaccine delivery, which is an easy-to-use, low-invasive, and painless method, has the potential to overcome certain issues associated with injectable vaccination. Adjuvants, which are used for enhancement of immune responses with vaccine, have not been developed for the transcutaneous vaccine. Therefore, it is desired to discover an effective and safe adjuvant available for transcutaneous delivery. In vivo</I> imaging technology can greatly contribute to kinetic analysis of adjuvants in the skin to predict their effect and safety. The purpose of this study is to image the adjuvants administrated transcutaneously in the mouse skin tissue sections by Imaging Mass Spectrometry (IMS) using MALDI (Matrix assisted laser desorption/ionization) method, which is unnecessary to label and can measure the distribution of the object. This study develop the protocol of IMS. Experimentally, ionization of K3-ODN on sample plate was examined. In this study, ATT was used as a matrix and spermine was used as a matrix additive. Ionization of K3-ODN in mouse skin tissue have been succeeded using this mixed solution. In future works, imaging mass spectrometry will be performed to obtain the ion images of K3-ODN in the tissue.