3E-L-1230 PDF
1) A Novel Lipid Screening Platform Allowing a Complete Solution for Lipidomics Research; 2) Biopharmaceutical Analytics : Evolution and Revolution
We utilized a specially designed kit for a simplified sample extraction and preparation of a serum matrix following the protocols provided. An LC-DMS (differential mobility spectrometry) -QTRAP® System (SCIEX) was used for targeted profiling of hundreds of lipid species from 10 different lipid classes allowing for comprehensive coverage. This system allowed for: 1. quantitative results for each lipid class as a sum of individual species; 2. mole percent composition was obtained computationally from lipid molecular species data; and 3. accurate lipid species compositions. The data was compared with historical data generated by alternative methods. Samples were quantitated using software accompanying the full solution which incorporates novel labeled internal standards available as a kit, developed for this platform (Avanti Lipids). Quantitative lipid species measurements were obtained from the following complex lipid classes: diacylglycerols (DAGs), triacylglycerols (TAGs), phosphatidylcholines (PCs), phosphatidylethanolamines (Pes), sphingomyelins (SMs), lysophosphatidylcholines (LPCs) and lysophosphatidylethanolamines (LPEs), free fatty acids (FFAs), cholesteryl esters (CEs) and ceramides (CERs). Covering these classes required a multi-injection approach but data can be collected in short, fast gradients up 5 minutes run time per injection (for three injections). The three injections classes covered TAGs and SMs, CEs and DAGs and FFAs, PE/PCs and their lyso components.