演題概要

オーラルセッション

第3日 6月19日(金) 16:25~16:45 B会場(102)

誘導体化イメージング質量分析法による担がん組織のアミノ酸分布の可視化

(1味の素2慶大医)
o東江咲乃1杉浦悠毅2久保亜紀子2大村光代2唐川幸聖1水越利巳1米田純也1宮野博1野口泰志1小林幹1加部泰明2末松誠2

MALDI-imaging mass spectrometry (IMS) combined with capillary electrophoresis-assisted mass spectrometry (CE/MS) serves as a method to provide semi-quantitative and spatial information of small molecular metabolites in tissue slices. However, not all metabolites including amino acids have fully been visualized, because of low ionization efficiency in MALDI mass spectrometry. In this presentation, I will describe a novel method for semi-quantitative MALDI-IMS to visualize multiple amino acids in a single tissue slice and the application of this technique to the study for cancer metabolism.