演題概要

ポスター発表

第2日 6月18日(木)  P会場

質量分析法を用いたα, β-不飽和カルボニル化合物の細胞内解毒機構の解明

(1武庫女大薬2理研3横市大)
o堀山志朱代1本田千恵1市川厚1畑井麻友子1吉川紀子1中村一基1國友勝1伊達沙智子2升島努2高山光男3

Aqueous cigarette smoke extract (CSE) contains many oxidants which can cause injury to various cells. We have previously detected methyl vinyl ketone (MVK), an α, β-unsaturated ketone, as an active ingredient in CSE, and also showed that MVK as well as CSE irreversibly modifies glutathione (GSH), an important antioxidant, in mouse melanoma cells, resulting in its rapid depletion. In addition, we have found that other reactive and toxic α, β-unsaturated aldehydes present in CSE, such as acrolein (ACR) and crotonaldehyde (CA), similarly react with GSH to form conjugates, which are further reduced to alcohols by aldose reductase in the cell. The mass spectral peaks at m/z 380 and m/z 366 corresponding to the mass of their alcohols were not detected when the cell were absent3).
In this study, we measured the intracellular and extracellular concentration of the conjugates with GSH and their reduced form (alcohols) to elucidate the cellular metabolism of the GSH conjugates of α, β-unsaturated carbonyl compounds, MVK, ACR and CA.