演題概要

ポスター発表

第２日　６月１８日（木）　　P会場

2P-19　　PDF

新規フラグメンテーション法”気相イオン水素付着解離(G-HAD)”の開発

(¹島津・ ²同志社大)
^o高橋秀典¹・ 関谷禎規¹・ 西風隆司¹・ 小寺慶¹・ 岩本慎一¹・ 和田元²・ 田中耕一¹

Hydrogen attachment dissociation of gas-phase ions (G-HAD) was observed in a quadrupole ion trap mass spectrometry. The hydrogen radical was injected into the ion trap to react with isolated peptide ions. The G-HAD spectra showed extensive backbone cleavage of both protonated and deprotonated ions in the form of a-, c-, x- and z- type ions. The cracking efficiency of molecular hydrogen and the flux density of injected hydrogen radical were greater than 50% and 5×10¹⁴ atoms/cm²s^-1 at the operating conditions, respectively. After irradiation with hydrogen radical, supplemental activation was applied to undissociated precursor ions to enhance G-HAD efficiency.