演題概要

ポスター発表

第1日 6月17日(水)  P会場

UFMS (ultra-fast mass spectrometry)を用いた出芽酵母1遺伝子破壊株29株の中心代謝酵素発現量プロファイリング

(1阪大情報2島津)
o松田史生1小倉泰郎2富田淳美1平野一郎2清水浩1

A quantitative proteome analysis was conducted to investigate expression profiles of carbon central metabolism-related enzymes in single gene deletion mutants of Saccharomyces cerevisiae by ultra-fast mass spectrometry. Levels of 104 enzymes in 29 mutant strains of S. cerevisiae were successfully determined by 261 SRM assay runs using nanoLC-ESI-MS/MS (Prominence nano + LCMS-8040). A comparison of the enzyme levels among S. cerevisiae mutants showed a dynamic change of enzyme expression levels by global and local regulation mechanisms.