1P-37 PDF
組換え糖転移酵素POMGnT1のO-結合型糖鎖解析
Protein O-mannose β-1,2-N-acetylglucosaminyltransferase 1 (POMGnT1) catalyzes the addition of N-acetylglucosamine (GlcNAc) to O-linked mannose (Man) to give GlcNAcβ1-2Manα-Ser/Thr. To investigate the glycan structure and modification site of POMGnT1, we analyzed the tryptic peptides of POMGnT1 by LC-MALDI-TOF/TOF system and detected the glycopeptides containing mucin-type O-linked glycan.