演題概要

ポスター発表

第1日 6月17日(水)  P会場

ネイティブマススペクトロメトリーによる金属蛋白質Ribonuclease H1の解析

(1阪大蛋白研2阪大院工3CIGB4JEOL)
o安東友繁1Tannous, Elias2Fernandez-de-cossio, Jorge3田村淳4金谷茂則2高尾敏文1

To reveal the protein complexes and protein interaction with other molecules such as DNA, organic compounds, or metal ions etc. is very important to understand their structure and function. Native mass spectrometry (Native MS) is a technique that allows measurement of protein complexes in native state. Native MS is normally achieved in combination with electrospray ionization, and has been applied for high-molecular-weight protein complexes with high sensitivity, and is, especially, useful for determining the stoichiometry of components.