1P-28 PDF
新型MALDIタンデムTOFMSを用いたMALDIイメージングにおけるマトリックス蒸着条件の検討
Sublimation technique for coating matrices is known to generate highly uniform and fine crystals of matrices, which enables to visualize precise distribution of targeted molecule in MALDI imaging. A thickness of coated matrix is necessary to be controlled to achieve high reproducibility. We developed a new sublimation technique, in which the thickness of matrix is monitored in real time by variation of laser light transmission, and it has been applied to Microscope mass spectrometer, in which AP-MALDI is incorporated. On the other hand, recently we developed a new MALDI-tandem TOFMS that has high resolution in MS/MS with a high energy CID at 20keV. We will report an experimental condition of the sublimation technique for MALDI imaging and applications taken by the new instrument.