The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 3, May 13（Wed.）　　Poster (1008/09)

3P-38　　PDF

Applying MALDI-Imaging Mass Spectrometry for the studies of murine olfactory deprivation and regeneration model

(1Doshisha Univ., 2Bruker)
oTakashi Nirasawa2, Daiki Kameyama1, Takuya Tomono1, Yumiko Toyama1, Ryo Kajita2, Nobuto Kakuda1, Masaya Ikegawa1

The olfactory bulb (OB) is the first relay on the olfactory sensory pathway and the target of the neural progenitor cells generated in the subventricular zone (SVZ) lining the lateral ventricles and which migrate along the rostral extension of the SVZ, also called the rostral migratory stream (RMS). Here we generate murine olfactory deprivation model by surgical unilateral nostril closure with a single intraperitoneal administration of Methimazole (MT), a drug for hyperthyroidism with a unique side effect of temporary and reversible olfactory epithelium (OE) depletion. On this model, we have defined proteomic basis of OB-RMS-SVZ axis using high speed MALDI imaging mass spectrometry.