The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
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Day 3, May 13（Wed.）　　Poster (1008/09)

3P-20　　PDF

Determination of Disulfide Bonding Patterns of K+-Channel Toxin-Like Peptides from the Venom of the Scorpion Liocheles australasiae

(Kyoto Univ.)
Naoya Mitani, Mitsuki Hirota, oMasahiro Miyashita, Yoshiaki Nakagawa, Hisashi Miyagawa

Scorpion venom contains peptides that have multiple disulfide bonds. Many of them are known to act on ion channels, such as Na+ and K+ channels. The transcriptome analysis of the venom gland of the scorpion Liocheles australasiae suggested the existence of various K+-channel toxin (KTx)-like peptides in the venom. In this study, we synthesized two KTx-like peptides (La-αKTx2 and La-κKTx4) to confirm their identity and determined their disulfide bonding patterns by enzymatic digestion and mass spectrometric analysis.