The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 3, May 13（Wed.）　　Poster (1008/09)

3P-17　　PDF

Characterization of Derivatized Carbohydrates Using High Resolution Cyclic IMS and Tandem-IMS Techniques

(1Georgia Tech, 2Waters - Pleasanton, 3Waters - Wilmslow, 4Nihon Waters K.K.)
Kristin Mckenna1, Andrew Baker2, Martin Palmer3, Dale Cooper-shepherd3, Facundo Fernandez1, oMasayuki Kubota4

Small sugars represent an interesting class of molecules that are difficult to characterize using conventional ion mobility spectrometry (IMS). By adding a suitable covalent shift reagent, differences in collisional cross section (CCS) are amplified, allowing for better identification and characterization. 3-carboxy-5-nitrophenylboronic acid (3C5NBA) reacts with hydroxyl groups on the sugars to form bidentate derivatives depending on the available cis-diol reaction sites defined by the sugar composition, carbohydrate linkage, and conformation. High resolution IMS using a variable path length mobility separator and tandem-IMS strategies (further separation of mobility-selected sub-populations) can be used to probe the complex mixture of isomeric carbohydrate derivatives.