Poster Presentations
- Day 3, May 13（Wed.） Poster (1008/09)
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3P-10 PDF
LC/MS analysis of oligonucleotides using polymer-based HILIC column having diol group
For development or quality control of oligonucleotide medicines, it is required to analyze them with high sensitivity and high selectivity using LC/MS. Though ion-pair reverse-phase chromatography have been used conventionally, there is a problem that ion-pair reagent tend to remain in LC/MS instrument. In order to solve this issue, a HILIC column Shodex® HILICpak® VN-50 2D with polyvinyl alcohol base packing material modified with diol group was used for the LC. Aqueous solution including ammonium formate / acetonitrile was used as eluent and ESI-MS was used for detection. As a result, it was confirmed to obtain good separation of 2-20mer DNA. This method enables the analysis of oligonucleotides without ion-pair reagent.