Abstract

Poster Presentations

Day 3, May 13（Wed.）　　Poster (1008/09)

3P-09　　PDF

Development of a Novel Mass Spectrometer with High Resolving Power for LC/MS Analyses of Biological Molecules

(¹Shimadzu, ²Shimadzu, ³SRL)
^oHiroyuki Miura¹, Yusuke Tateishi¹, Hideaki Izumi¹, Osamu Furuhashi¹, Junichi Taniguchi¹, Kiyoshi Ogawa¹, Hiromasa Maruno¹, Takao Nakatomi², Ryo Fujita³, Vyacheslav Shchepunov³, Michael Rignall³, Stuart Harley³, Hiroaki Waki³, Jeff Chadbourne³

We have developed a novel multi-turn TOF-MS (MTOF). This system avoided the "overtaking " that has been encountered in conventional MTOF, and achieved high resolving power while keeping its size compact. We constructed LC-MTOF-MS and evaluated the performance. Mass resolving power higher than 150k (FWHM) and instrument detection limit of approximately 3 amol were achieved. Furthermore, MS analyses of biological molecules were performed. Isotopic peaks of cytochrome c (approximately 12kDa, 8+) were clearly separated.