The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 3, May 13（Wed.）　　Poster (1008/09)

3P-02　　PDF

Development of Electrospray-Assisted Laser Desorption/Ionization Source for Imaging of Intracellular Molecules

(1Osaka Univ., 2Osaka Univ., 3Shimadzu, 4Osaka Univ.)
oYuto Miyoshi1, Hiroki Kannen1,3, Hisanao Hazama1, Kunio Awazu1,2,4

Imaging of intracellular molecules is important analytical technique to understand dynamics of proteins and metabolites in a cell. In previous study, using mass spectrometry imaging, high spatial resolution (1 μm) was achieved. However, sensitivity of ions is not sufficient. In order to acquire clear and detailed image, it’s necessary to increase amount of ions. In this experiment, to improve ionization efficiency, ELDI (Electrospray-assisted laser desorption/ionization; ELDI) source combining laser desorption ionization (LDI) and electrospray ionization (ESI) under atmospheric pressure and optical system was constructed. Using ELDI, crystal violet (CV) was measured and the effectiveness of ELDI source was evaluated.