Luncheon Seminars
- Day 3, May 13（Wed.） 12:00-13:00 Room C (1101/02)
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3C-L-1200 PDF
New sample prep products suitable for Lipidomics, and Lipid Annotator software
This lunch on seminar introduces new sample prep product for lipidomics and MassHunter Lipid Annotator software to identify lipids comprehensively from Agilent.
Agilent is now manufacturing Captiva EMR Lipid to remove lipids form biological samples to clean up non-lipid analytes. Using the same technology, new sample prep product which clean up lipids by removing non-lipid substances was developed. It is suitable for lipidomics analysis. The feature, way to use and application data of this new product is introduced.
A major challenge in mass spectrometry-based lipidomics is the comprehensive characterization of a large and diverse set of lipid species, spanning a wide concentration range within a biological sample. While shotgun lipidomics has advanced the field of lipid analysis, it suffers from limitations including the failure to distinguish isobaric species, which may be of biological importance and reduced dynamic range due to ionization suppression. This led to chromatography based lipid profiling approaches using ultra high-performance liquid chromatography (UHPLC) coupled to high resolution mass spectrometry (HR-MS). We will show the fully automated iterative MS/MS and Lipid Annotator software as part of a comprehensive lipidomics workflow.