The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Oral Sessions

Day 3, May 13（Wed.）　15:50-16:10　Room A (Main Hall)

3A-O13-1550　　PDF

Structure estimation of gram-negative gut bacterial lipid A using untargeted analysis

(1Osaka Univ., 2RIKEN IMS, 3Keio Univ.)
oNobuyuki Okahashi1,2, Fumio Matsuda1, Makoto Arita2,3

A method for the analysis of lipid A, an important immune activator of gram-negative bacteria, was developed by using LC-QTOF-MS. The sharp peak of lipid A was observed by employing a metal-free column and high pH basic solvent. MS/MS spectra assignment enabled the structure estimation of lipid A. This method was successfully applied to the analysis of in vitro cultured gram-negative gut bacteria strains. The results suggest the structural diversity of lipid A.