The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

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Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

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Luncheon Seminars (Day1, Day2, Day3)

Oral Sessions

Day 3, May 13（Wed.）　15:10-15:30　Room A (Main Hall)

3A-O13-1510　　PDF

Wide-range metabolomic analysis of hydrophilic metabolites by next-generation ion chromatography high resolution tandem mass spectrometry

(1Kyushu Univ., 2Thermo Fisher)
oMasatomo Takahashi1, Yoshihiro Izumi1, Takahiro Suzuki2, Kohta Nakatani1, Kosuke Hata1, Kentaro Takahara2, Takeshi Bamba1

Almost all intermediates of essential metabolic pathways to maintain biological activities are cationic, zwitterionic, or anionic metabolites. Therefore, a profiling for these ionic metabolites provides valuable insight towards understanding metabolic regulation. To date, we have developed methods for the analyses of anionic metabolites and cationic metabolites, respectively, using ion chromatography high resolution tandem mass spectrometer (IC/HRMS/MS). However, in conventional IC system, many of zwitterionic metabolites such as amino acids and their derivatives are removed by a process that uses suppressors to convert the high salt eluent to pure water (i.e., desalting). The aim of this study is to develop an advanced analytical system for wide-range metabolomic analysis of cationic, zwitterionic, and anionic metabolites by IC/HRMS/MS with novel cationic and anionic suppressors.