The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 2, May 12（Tue.）　　Poster (1008/09)

2P-24　　PDF

13C-Metabolic flux analysis of HL-60 myeloid leukemia cells using mass spectrometry

(Osaka Univ.)
oTakeo Taniguchi, Nobuyuki Okahashi, Fumio Matsuda

The 13C-metablic flux analysis using mass spectrometry was used to investigate metabolic flux distributions in differentiated and non-differentiated HL-60 myeloid leukemia cells. A time course analysis of cell numbers, medium components and 13C-labeling kinetics of the intracellular metabolites confirmed that 13C-MFA could be applied for the immune cells. Flux distributions in the central carbon metabolism suggested that differentiated HL-60 cells shifted the metabolism from glucose to amino acids-dependent.