The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 2, May 12（Tue.）　　Poster (1008/09)

2P-18　　PDF

Mass Spectrometric Analysis of Volatile Metabolites in Aspergillus nidulans for Sesquiterpene Biosynthetic Pathway Elucidation VIII. AN3280 Gene Expression-enforced Strains: LO8925 and LO8910HA-pyrG2.

(1Nara Women's Univ., 2Nagoya Univ., 3Nara Women's Univ.)
oMariko Hattori1, Tomohiro Akashi2, Takae Takeuchi1,3

Microbial volatile organic compounds (MVOCs) emitted from Aspergillus nidulans were investigated using Head Space-SPME/GC/MS, and the effects of modified expression of some sesquiterpene biosynthesis genes in the genome of A. nidulans were analyzed. In strain LO8910HA-pyrG2, a part of AN3280 gene upstream was eliminated from LO8910. The eliminated region seemed to surpress transcription in the parental strain LO8910. 7-epi-neopetasone, which is an intermediate of PR-toxin, was detected in LO8910HA-pyrG2 as reported previously in LO8925. We also found that LO8910HA-pyrG2 can produce a new sesquiterpene MVOC.