Abstract

Poster Presentations

Day 2, May 12（Tue.）　　Poster (1008/09)

2P-13　　PDF

Identification of aminopropyl groups bound in the C-terminal region of branched-chain polyamine synthase (BpsA by mass spectrometry

(¹Kobe Univ., ²Kobe Univ., ³Kobe Univ., ⁴Kwansei Gakuin Univ., ⁵Kobe Pharm. Univ.)
^oKen-ichi Yoshino^1,2, Ryota Hidese³, Shinsuke Fujiwara⁴, Atsuko Takeuchi⁵

In the present study, LC-MS/MS analysis of Themococcus kodakarensis BpsA (Tk-BpsA)-dcSAM reaction product was conducted, revealing two aminopropyl groups bound to either neighboring two of five acidic residues (E³³⁹, D³⁴², D³⁴³, E³⁴⁴, and E³⁴⁵) in C-terminal flexible region.