The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 2, May 12（Tue.）　　Poster (1008/09)

2P-08　　PDF

Improvement of 13C Metabolic Flux Analysis by measuring cell components.

(Osaka Univ.)
oFuta Yatabe, Nobuyuki Okahashi, Fumio Matsuda

The cell composition measurement using GC-MS was applied to budding yeast, a model eukaryote organism. Amount of 14 proteinous amino acids in the four budding yeast strains were successfully quantified by GC-MS. In the literature values, the composition of methionine, alanine, Asx (=asparagine + aspartate), lysine and valine were overestimated as compared with the measured values. BY4947 and RedStar showed high protein ratios in the dry cell weight. Flux distributions calculated using the amino acid composition of literature values and the measured amino acids were compared.