The Mass Spectrometry society of Japan - The 68th Annual Conference on Mass Spectrometry, Japan
Japanese

Abstract

Table of contents

Timetable

Plenary Lectures

Oral Sessions (Day1, Day2, Day3)

Poster Presentations (Day1, Day2, Day3)

Workshops

Luncheon Seminars (Day1, Day2, Day3)

Poster Presentations

Day 2, May 12（Tue.）　　Poster (1008/09)

2P-06　　PDF

Evaluation of enhanced sensitivity mass spectrometry imaging using DIUTHAME chip

(1Hamamatsu Photonics, 2GPI)
oMasahiro Kotani1, Takayuki Ohmura1, Akira Tashiro1, Yasuhide Naito2

We developed DIUTHAME (Desorption Ionization Using Through Hole Alumina MEmbrane) instead of matrix compounds in MALDI. Matrix-free DIUTHAME doesn’t have proton donating function in principle, so that it sometimes lower signal than MALDI. This study aims to examine the signal intensity can be improved by coating the DIUTHAME chip with a cationizing agent. Comparing the MS imaging results of the mouse brain section between with and without NaCl coating on DIUTHAME chip, it was observed that the signal intensity increased at [M+Na]+ of phosphatidylcholine with the NaCl-coated chip. This result was presumably caused by integrating the signals separately detected as protonated, sodiated, and potassiated molecules into sodium cationized one. it was suggested that the DIUTHAME chips coated with the cationizing agents can improve the signal intensity while utilizing the characteristics advantages of DIUTHAME.