Abstract

Oral Sessions

Day 2, May 12（Tue.）　10:25-10:45　Room A (Main Hall)

2A-O4-1025　　PDF

Imaging mass spectrometry for Alzheimer's disease (AD) brain using DIUTHAME

(¹Doshisha Univ., ²Hamamatsu Photonics, ³Bruker, ⁴TMIG, ⁵GPI)
^oYumiko Toyama¹, Yume Mukasa¹, Inori Shintani¹, Takayuki Ohmura², Masahiro Kotani², Akira Tashiro², Takashi Nirasawa³, Nobuto Kakuda¹, Shigeo Murayama⁴, Yasuhide Naito⁵, Masaya Ikegawa¹

Neuropathology of Alzheimer’s disease (AD) is characterized by the accumulation and aggregation of Amyloid β (Aβ) peptides into extracellular plaques of the brain. Increasing lines of evidence support a direct involvement of cholesterol in AD pathogenesis as well. For the rapid and reproducible analysis of multifactorial autopsy brains, here we adopt a novel matrix‐free laser desorption/ionization method, Desorption Ionization Using Thorough Hole Alumina Membrane (DIUTHAME) in combination with conventional MALDI-IMS on human autopsy brain tissues to obtain a comprehensive molecular mapping.
Frozen tissue sections were cut on a cryostat at a 20 µm thickness onto ITO glass slides. Spectra were acquired using the rapifleX MALDI Tissuetyper, whereas ions were detected with spatial resolution of 50 µm.
We have succeeded in obtaining low molecular imaging with DIUTHAME system for the brains from AD in contrast with non-pathological aged subjects. In order to gain sensitivity, we utilized TM-sprayer for the hydration of the surface of the DIUTHAME cassette. From AD and non-pathological brain section, m/z at 147 for grey matter dominant and m/z at 184 for white matter dominant. These can be annotated to cholesterol fragment and phosphatidylcholine fragments, respectively.